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The best way to be sure that you are pitching enough healthy yeast is to make a starter. I use a stir plate to encourage aerobic fementation as that's the time where the yeast reproduce making lots of fresh, healthy, new cells.
Here's a picture of my yeast starter for tomorrow's (actually today's) brew. It's about 4.5l of wort made with DME to an OG of 1.040 with a load of slurry from an earlier, unused starter added about 4 hours ago.
You can see my refractometer standing upright at the back of my stirplate. It's a great piece of kit that every brewer should have.
The cold weather came in handy tonight as I was able to bring the temperature of the wort from boiling to pitching temperature within minutes just by dunking it in a bucket of water that was sitting by the back door. After I broke the ice on it it was a perfect wort chiller!
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As the source of the slurry had been sitting in the kitchen for a couple of weeks I did a quick check on the health of the cells prior to pitching. The photo to the right shows that the yeast cells are healthy. There's a little debris around but no nasties that I can see.
I'll do a viability check on the starter (using methylene blue as an indicator) prior to pitching into my wort. It it's infected (which I doubt, looking at the activity in the flask right now) I'll pitch a fresh tube of WLP002 and aerate like mad.
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